Excess colloidal gold migrates across to the control line, where an antibody directed to the gold-labeled antibody binds to it. In a positive sample, this generates a pink to purple colored reaction line in window 2.A second antibody specific to the antigen binds the antigen/antibody gold complex at the test line. After the sample and buffer are added to the sample pad (window 1), any antigens in the sample migrate across a nitrocellulose membrane along with the gold-labeled antibody (conjugate) and form an antigen/antibody gold complex.WITNESS ® tests perform well without refrigeration using an antigen-specific antibody labeled with colloidal gold. This technology is also sometimes referred to as lateral flow or rapid immunochromatography. The WITNESS ® format is referred to as Rapid ImmunoMigration (RIM ™). WITNESS ® Heartworm detects the presence of adult Dirofilaria immitis antigens in dog or cat blood.
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